Sevplate
Why problem 1 goes first
The next six problems only receive wells that passed the QC gate.
Problem 1 asks two things at once: against the negative control, is this well active, and can this plate and batch be used? Problems 2 through 7 only receive wells that passed that gate.
What goes in
Exactly one source per well: a numeric profile, or an image. With it: well identity, batch, replicate group, and the plate map. On the map each well has a role: negative control, treatment, positive control, or empty. The modality is RNA, protein, or image.
What comes out
- Plate and batch: pass or fail.
- Each well: pass, fail, or exclude.
- Each replicate group: active, inactive, or ambiguous when replicates disagree in direction.
- The accepted-well list: only these wells move on.